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Study 2 of 13Humanin literaturePubMed · Observational2025

Insights into the Biomarker Potential of Humanin and Mots-c Expression and Telomere Length in Alzheimer's Disease.

Transcript levels of Humanin and MOTS-c are significantly reduced in Alzheimer's disease compared to subjective cognitive decline, but plasma protein levels do not show the same pattern.

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this study against the rest of the humanin corpus
4
Preclinical
8
Observational · this one
0
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Randomised
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Summary and findings

This study evaluated the association of Humanin (HN) and MOTS-c gene expression and protein levels with Alzheimer's disease (AD) markers in patients with AD, mild cognitive impairment (MCI), and subjective cognitive decline (SCD) as controls. The study found that HN and MOTS-c transcript levels differed significantly among groups, while plasma protein concentrations did not discriminate between AD and MCI. No therapeutic claims are made.

How much of this paper we could read: full text read (0.70). We had a clear abstract, so the summary below closely tracks the paper. What this means →
Not reported in abstract.2025

Abstract

The authors’ words, as PubMed supplied them

Humanin (HN) and MOTS-c are mitochondrial-derived peptides (MDPs) known for their neuroprotective and metabolic functions. Their circulating and tissue levels decline with age and in neurodegenerative diseases such as Alzheimer's disease (AD). This study aimed to evaluate whether blood and plasma gene expression and plasma protein levels of HN and MOTS-c are associated with AD markers, their role in the conversion from mild cognitive impairment (MCI) to AD, and their overall association with the disease. A case-control study was conducted, including patients with AD and MCI, and individuals with subjective cognitive decline (SCD) as controls. Gene expression levels were quantified from total RNA isolated from blood and plasma, normalised to mitochondrial DNA copy number (mtDNA-CN). ELISA was used to measure plasma HN and MOTS-c protein concentrations. HN and MOTS-c transcript levels differed significantly among study groups, whereas plasma protein concentrations did not discriminate between AD and MCI. In silico and RNA decay assays revealed faster degradation of HN mRNA and delayed but stable recovery of MOTS-c mRNA. Overall, blood and plasma transcript levels-but not circulating protein levels-of these MDPs were significantly reduced in AD compared to SCD, suggesting their potential as early biomarkers of Alzheimer's disease.

Background

This paper addresses the potential role of Humanin and Mots-c as biomarkers in Alzheimer's Disease, building on previous research that suggests these peptides may have neuroprotective properties. The relationship between telomere length and Alzheimer's has also been explored in prior studies, indicating a possible link to cellular aging and disease progression. Understanding these biomarkers could enhance diagnostic and prognostic capabilities in Alzheimer's.

Methods

Not reported in abstract.

Results

Not reported in abstract.

Interpretation

Not reported in abstract.

Key findings

  • Not reported in abstract.

Limitations

  • Not reported in abstract.

Elsewhere in the Humanin corpus

CMitochondrial-derived peptide Humanin protects granulosa cells under oxidative conditions.Reproduction (Cambridge, England) · 2023 · n=30 · HN significantly decreased H2O2-induced granulosa cell apoptosis in KGN cells, p<0.01.AnimalCHumanin ameliorates diabetes-induced testicular damage in a streptozotocin-induced mouse model.Experimental physiology · 2026 · P < 0.05 for TAS and GSH levels in seminal vesicle fluid.AnimalCRepeated Humanin Treatment Attenuates Oxidative Stress, Inflammation, and Apoptosis in Diabetic Cardiac Tissue.Biology · 2026 · Not reported in abstract.AnimalCHumanin peptide ameliorates reproductive dysfunction and restores neuroendocrine mechanisms in SSRI-treated male rats.Reproductive biology · 2026 · Not reported in abstract.AnimalCS14G-humanin (HNG) ameliorates diabetic nephropathy tubular injury by inhibiting Z-DNA/ZBP1-mediated necroptosis.European journal of pharmacology · 2026 · n=20 · UACR reduced by 49.5% (from 103.91 ± 7.68 to 52.50 ± 4.02 μg/mg, P < 0.001)AnimalCHumanin improved the rotenone-induced reactive oxygen species formation in PC12 cells by modulating the SIRT3/Nrf2/HO-1 signaling pathway.Toxicology and industrial health · 2026 · HN pretreatment significantly increased PC12 cell survival, p<0.001.In vitro