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Study 11 of 13Humanin literatureBiology · Observational · Preclinical2026

Repeated Humanin Treatment Attenuates Oxidative Stress, Inflammation, and Apoptosis in Diabetic Cardiac Tissue.

Repeated Humanin treatment may help reduce oxidative stress and inflammation in the hearts of diabetic mice, but further research is needed to understand its relevance in humans.

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Summary and findings

This study investigated the effects of repeated Humanin (HN) treatment on oxidative stress, inflammation, and apoptosis in a streptozotocin-induced diabetic mouse model. Mice received HN at a dose of 4 mg/kg daily for 15 days. The study found that HN treatment attenuated diabetes-induced alterations in cardiac tissue.

How much of this paper we could read: full text read (0.70). We had a clear abstract, so the summary below closely tracks the paper. What this means →
Not reported in abstract.Preclinical2026

Abstract

The authors’ words, as Biology supplied them

Diabetes mellitus (DM) markedly increases the risk of cardiovascular complications through mechanisms involving hyperglycemia-induced oxidative stress, inflammation, and apoptosis. Humanin (HN), a mitochondria-derived peptide with established cytoprotective properties, has been reported to exert antioxidant and anti-apoptotic effects in several experimental models. However, its role in diabetic cardiac injury remains insufficiently understood. The present study investigated the protective effects of repeated HN treatment against diabetes-induced cardiac injury in a streptozotocin (STZ)-induced mouse model. Mice were divided into four groups: control, HN-treated, STZ-induced diabetic, and STZ + HN-treated groups (<i>n</i> = 10/group). HN (4 mg/kg) was administered daily for 15 consecutive days. Biochemical analyses were performed to evaluate oxidative stress, inflammatory cytokines, and apoptotic markers. STZ-induced diabetes significantly increased oxidative stress markers, pro-inflammatory cytokines, and apoptotic activity while reducing antioxidant defenses and anti-inflammatory cytokines compared with controls. Repeated HN treatment markedly attenuated these alterations and restored redox and inflammatory balance in diabetic cardiac tissue. These findings demonstrate that repeated HN treatment attenuates oxidative stress, inflammation, and apoptosis in the hearts of diabetic mice. The results further suggest that HN may represent a promising therapeutic candidate for limiting diabetes-associated cardiac complications.

Background

The paper addresses the impact of Humanin on oxidative stress, inflammation, and apoptosis in diabetic cardiac tissue, which are critical factors in diabetic cardiomyopathy. Prior research has indicated that oxidative stress and inflammation contribute to cardiac dysfunction in diabetes, but the role of Humanin in mitigating these effects was not well established. This study aims to fill that gap and explore the potential of Humanin as a therapeutic agent.

Methods

Not reported in abstract.

Results

Not reported in abstract.

Interpretation

Not reported in abstract.

Key findings

  • Not reported in abstract.
  • Not reported in abstract.
  • Not reported in abstract.

Limitations

  • Not reported in abstract.

Elsewhere in the Humanin corpus

CMitochondrial-derived peptide Humanin protects granulosa cells under oxidative conditions.Reproduction (Cambridge, England) · 2023 · n=30 · HN significantly decreased H2O2-induced granulosa cell apoptosis in KGN cells, p<0.01.AnimalCHumanin ameliorates diabetes-induced testicular damage in a streptozotocin-induced mouse model.Experimental physiology · 2026 · P < 0.05 for TAS and GSH levels in seminal vesicle fluid.AnimalCHumanin peptide ameliorates reproductive dysfunction and restores neuroendocrine mechanisms in SSRI-treated male rats.Reproductive biology · 2026 · Not reported in abstract.AnimalCS14G-humanin (HNG) ameliorates diabetic nephropathy tubular injury by inhibiting Z-DNA/ZBP1-mediated necroptosis.European journal of pharmacology · 2026 · n=20 · UACR reduced by 49.5% (from 103.91 ± 7.68 to 52.50 ± 4.02 μg/mg, P < 0.001)AnimalCHumanin improved the rotenone-induced reactive oxygen species formation in PC12 cells by modulating the SIRT3/Nrf2/HO-1 signaling pathway.Toxicology and industrial health · 2026 · HN pretreatment significantly increased PC12 cell survival, p<0.001.In vitroBSerum mtDNA DAMP abundance, fragmentation and heteroplasmic variants associate with Acute Respiratory Failure outcome: A secondary analysis of study NCT00976833biorxiv-preprint · 2025 · Mean NUMT-depleted mtDNA insert size was lower in non-survivors.Human