Association of central α-klotho with dopaminergic and melanocortin receptors on feed intake regulation in chicken.
The study found that α-klotho influences feed intake in chickens through interactions with certain dopaminergic and melanocortin receptors, but the practical implications of these findings are not yet clear.
Where it sits
this study against the rest of the melanotan ii corpusSummary and findings
This study examined the association of central α-klotho with dopaminergic and melanocortin receptors in regulating feed intake in 264 meat-type chickens across six experiments. Various receptor antagonists were administered, and feed intake was measured up to 120 minutes post-injection. The results indicated that certain receptor antagonists influenced the α-klotho-induced reduction in feed intake.
Abstract
This study aimed to detemine association of central α-klotho with dopaminergic and melanocortin receptors on feed intake regulation in chicken. 264 meat-type chicken were included 6 experiments, each containing 4 groups. In experiment 1, the group (A) received injections of saline, group (B) SCH23390 (D1 receptor antagonist, 5 nmol), group (C) α-klotho (4 µg) and group (D) co-injection of the SCH23390 + α-klotho. In experiments 2-6, AMI-193 (D2 receptor antagonist, 5 nmol), NGB2904 (D3 receptor antagonist, 5 nmol), L-741,742 (D4 receptor antagonist, 5 nmol), SHU9119 (nonselective MC3/MC4 receptor antagonist, 0.5 nmol) and MCL0020 (selective MC4 receptor antagonist, 0.5 nmol), instead of the SCH23390. Then feed intake determined up to 120 minutes post injection. Based on the results, D1 receptor antagonist decreased α-klotho-induced hypophagic effect compared to control group (P < 0.05). Also, D2 receptor antagonist decreased α-klotho-induced hypophagic effect compared to control group (P < 0.05). Co-injection of the D3 receptor antagonist + α-klotho had no effect on α-klotho-induced hypophagia (P > 0.05). Co-injection of the D4 receptor antagonist + α-klotho had no effect on α-klotho-induced hypophagia (P > 0.05). Co-injection of the nonselective MC3/MC4 receptor antagonist decreased α-klotho-induced hypophagic effect compared to control group (P < 0.05). Co-injection of the selective MC4 receptor antagonist decreased α-klotho-induced hypophagic effect compared to control group (P < 0.05). Findings suggested α-klotho-induced hypophagia mediates by D1 and D2 dopaminergic as well as MC3 and MC4 melanocortin receptors in neonatal broiler chicken.