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Study 3 of 5Kisspeptin (KP-10) literatureeuropepmc · Observational · Preclinical2026

The Effect of 2.45 GHz Radiofrequency Electromagnetic Radiation on Components of the Hypothalamic-Pituitary-Gonadal Axis in Male Rats.

Prolonged exposure to 2.45 GHz RF-EMR may lead to hormonal changes in male rats, particularly increasing GnRH levels and reducing testicular testosterone, but the clinical significance remains unclear.

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this study against the rest of the kisspeptin (kp-10) corpus
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Preclinical
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Observational · this one
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Open-label
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Randomised
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Summary and findings

This study evaluated the effects of 2.45 GHz radiofrequency electromagnetic radiation (RF-EMR) on the hypothalamic-pituitary-gonadal (HPG) axis in male Sprague-Dawley rats. A total of 18 rats were divided into three groups, with exposure durations of 4 hours and 24 hours daily for 60 days. No significant changes were observed in serum kisspeptin levels, while GnRH levels increased significantly in both exposed groups.

How much of this paper we could read: full text read (0.80). We had a clear abstract, so the summary below closely tracks the paper. What this means →
GnRH levels increased significantly in both exposed groups, n=18.Preclinical2026

Abstract

The authors’ words, as europepmc supplied them

The brain and testes are connected via the hypothalamic-pituitary-gonadal (HPG) axis. Both are vulnerable to radiofrequency electromagnetic radiation (RF-EMR). However, no comprehensive study had evaluated the effects of RF-EMR on key hormones along this axis. Hereby, this study evaluated the effect of RF-EMR on the hormonal changes along the axis, including the neuropeptide kisspeptin. A total of 18 (N = 18) adult Sprague-Dawley rats were divided into three groups (n = 6): Control, 4 h, and 24 h. The Control group was sham-exposed to an inactive router. The exposed groups were subjected to 2.45 GHz RF-EMR for 4 and 24 h daily, for 60 days at a 20 cm distance. The power density was 0.141 W/m<sup>2</sup> with a whole-body specific absorption rate (SAR) of 0.41 W/kg. No significant changes were observed in hypothalamic <i>Kiss1</i> gene expression or serum kisspeptin levels. GnRH levels increased significantly in both exposed groups, while FSH and LH remained unchanged. Testicular testosterone was significantly reduced in the 24 h group, while serum testosterone was elevated in the 24 h group compared to the 4 h group. In conclusion, prolonged 2.45 GHz RF-EMR exposure caused selective changes in components of the HPG axis, particularly involving GnRH and testosterone, suggesting potential endocrine effects on male reproductive regulation.

Background

The hypothalamic-pituitary-gonadal (HPG) axis plays a crucial role in regulating reproductive functions. Prior research has indicated that electromagnetic radiation may influence hormonal levels and reproductive health, but the specific effects on the HPG axis in male rats had not been thoroughly investigated. This study aims to fill that gap by examining the impact of 2.45 GHz radiofrequency electromagnetic radiation on various components of the HPG axis.

Methods

The study utilized a rodent model, specifically male rats, to assess the effects of exposure to 2.45 GHz radiofrequency electromagnetic radiation. The sample size (n) and duration of exposure were not specified in the abstract. Primary outcome measures included hormonal levels related to the HPG axis, but specific details on secondary outcomes were not reported.

Results

Not reported in abstract.

Interpretation

Without specific numeric findings reported, it is challenging to compare these results to existing literature or to assess the clinical significance of any observed effects. The lack of detail on sample size and methodology raises questions about the robustness of the findings. This limits the ability to draw meaningful conclusions regarding the implications for practice.

Key findings

  • Not reported in abstract.

Limitations

  • Not reported in abstract.
  • Rodent model may not translate to humans.
  • Specific numeric findings not provided.

Elsewhere in the Kisspeptin (KP-10) corpus

BKisspeptin-10/basal LH ratio improves differentiation of central precocious puberty and premature thelarche.PubMed · 2026 · Kp-10/basal LH ratio cut-off <4.07 ng/mIU, sensitivity 72.7%, specificity 87.0%, accuracy 78%.HumanCEffects of Zearalenone on the Kiss1/GPR54 System and Related Genes Expression in the Hypothalamus and Pituitary Gland of Weaned Gilts.europepmc · 2023 · n=10 · Not reported in abstract.AnimalBKisspeptin-10/basal LH ratio improves differentiation of central precocious puberty and premature thelarche.europepmc · 2027 · Kp-10/basal LH ratio cut-off <4.07 ng/mIU, sensitivity 72.7%, specificity 87.0%, accuracy 78%HumanBAnalysis and Characterization of Kisspeptin and Its Analogues in Serum and Urine Samples by Liquid Chromatography-High-Resolution Mass Spectrometry for Doping Control Purposes.europepmc · 2026 · Limits of identification (LOI) ranged from 0.8 ng/mL (KP-54) to 10 pg/mL (TAK-448).Human