Zein-Derived Peptides from Corn Promote the Proliferation of C2C12 Myoblasts via Crosstalk of mTORC1 and mTORC2 Signaling Pathways.
Zein-derived peptides increased C2C12 myoblast cell viability by 33.37 to 42.39% and reduced apoptosis from 34.08% to 28.96%, but these findings are based on in vitro studies.
Where it sits
this study against the rest of the igf-1 lr3 (long r3 igf-1) corpusSummary and findings
The study investigated the effects of zein-derived peptides on C2C12 myoblasts, focusing on their proliferative and anti-apoptotic activities. Zein peptides were administered at doses of 0.05-0.2 mg/mL. The findings indicated a significant increase in cell viability and a decrease in apoptosis rate.
Abstract
Dietary protein supplementation has emerged as a promising strategy in combating sarcopenia. Furthermore, searching for alternatives of animal proteins has been a hot topic. The present study aimed to investigate the effects of zein peptides on C2C12 myoblasts and explore their potential molecular mechanisms. The proliferative, cell cycle, and anti-apoptotic activities of zein peptides were evaluated. Peptidomics analysis and transcriptome sequencing were employed to explore the structure-activity relationship and underlying molecular mechanisms. The results indicated that zein peptides (0.05-0.2 mg/mL) exerted a significant proliferation-promoting impact on C2C12 cells, via increasing cell viability by 33.37 to 42.39%. Furthermore, zein peptides significantly increased S phase proportion and decreased the apoptosis rate from 34.08% (model group) to 28.96% in C2C12 cells. In addition, zein peptides exhibited a pronounced anti-apoptotic effect on C2C12 cells. Zein peptides are abundant in branch-chain amino acids, especially leucine. Transcriptomics analysis revealed that zein peptides can promote proliferation, accelerate cell cycle, and improve protein synthesis of muscle cells through mTORC1 and mTORC2 signaling pathways.
Background
The paper addresses the biological question of how zein-derived peptides from corn can influence muscle cell proliferation, specifically through the mTOR signaling pathways. Prior research has indicated that mTOR plays a crucial role in muscle growth and regeneration. Understanding the effects of these peptides could have implications for nutritional strategies aimed at enhancing muscle health.
Methods
Not reported in abstract.
Results
Not reported in abstract.
Interpretation
Not reported in abstract.
Key findings
- Not reported in abstract.
Limitations
- Not reported in abstract.