Oxytocin receptor Gαi signaling drives valence reassignment to facilitate reversal of social trauma-induced avoidance

The study highlights the importance of specific neuronal activity patterns in social fear extinction, suggesting potential avenues for addressing treatment-resistant social anxiety disorder.

Summary and findings

This study investigates the role of oxytocin receptor (OXTR)-expressing neurons in the caudal lateral septum in social fear extinction in mice. It examines the effects of chemogenetic silencing and pharmacological manipulation of Gαi and Gαq signaling pathways on social behavior. The findings indicate that specific neuronal activity patterns are crucial for adaptive learning during social fear extinction.

Abstract

Traumatic social experiences often generate social deficits that form the core symptom of psychopathologies like social anxiety disorder (SAD). The failure to overwrite these aversive social associations is a fundamental barrier to recovery from SAD. Using the mouse social fear conditioning (SFC) paradigm, we identify a discrete population of oxytocin receptor (OXTR)-expressing neurons in the caudal lateral septum (LScOXTR) that act as a critical hub for adaptive learning during social fear extinction. Using calcium imaging in mice undergoing extinction training, we show that successful valence reassignment is best predicted by the precise temporal inhibition of LScOXTR neurons during the contact phase of social investigation. This contact-associated suppression of LScOXTR neurons was absent in mice that failed to extinguish fear. Notably, successful extinction was also associated with enhanced oxitocinerg innervation within the LSc. Chemogenetic silencing of LScOXTR neurons prior to extinction impaired extinction, indicating that a specific temporal activity pattern of LScOXTR neurons is necessary for adaptive learning. Pharmacological dissection of downstream OXTR signaling revealed that selective activation of Gαi-coupled, but not Gαq-coupled, signaling accelerated extinction and promoted social approach. Our findings establish that extinction success is governed by the temporally precise, Gαi-mediated silencing of LScOXTR neurons during social contact, revealing a novel mechanism for adaptive social valence processing with direct implications for treatment-resistant SAD.

Limitations

Not reported in abstract.

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