Ultrasensitive low-density lipoprotein monitoring in early cardiovascular risk assessment via Cu-MOF-Apt/MoS₂ fluorescent nanosensor.
This study presents a new fluorescent sensor that can detect LDL levels as low as 0.0789 ng/mL, potentially improving early cardiovascular risk assessment.
Where it sits
this study against the rest of the ghk-cu corpusSummary and findings
A fluorescent aptamer sensor based on a copper-based metal-organic framework was developed to monitor low-density lipoprotein (LDL) levels. The sensor demonstrated a linear range of 0.1-10,000 ng/mL and an ultra-low detection limit of 0.0789 ng/mL. It showed good accuracy and precision in human serum samples.
Abstract
Cardiovascular disease (CVD) has emerged as the leading cause of mortality worldwide. Low-density lipoprotein (LDL), a critical serum biomarker for ASCVD diagnosis, plays a pivotal role in early cardiovascular disease detection and therapeutic monitoring. To address this need, we have developed a highly ultrasensitive fluorescent aptamer sensor based on a copper-based metal-organic framework (Cu-MOF) fluorescent probe. In this system, MoS₂ nanosheets can effectively quench the fluorescence of Cu-MOF via and fluorescence resonance energy transfer (FRET). Upon LDL binding, the aptamer interaction triggers the release of Cu-MOF-Apt from MoS<sub>2</sub>, restoring the fluorescence of Cu-MOF. Under the optimal conditions, the developed fluorescent sensor demonstrated broad linear range (0.1-10,000 ng/mL of LDL concentration), ultra-low detection limit (0.0789 ng/mL), excellent reproducibility, stability, and specificity. In addition, it showed good accuracy and precision in human serum samples (with relative errors of 0.09% ∼ 3.00% and relative standard deviations of 1.24% ∼ 3.74%), which can meet diagnostic standards. This work establishes a foundation for early CVD intervention and shows strong potential for point-of-care LDL monitoring, personalized cardiovascular therapy and precision medicine applications.
Background
Cardiovascular disease (CVD) is the leading cause of mortality globally, and low-density lipoprotein (LDL) is a critical biomarker for its diagnosis. Early detection of LDL levels is essential for effective therapeutic monitoring and intervention. This study presents a novel fluorescent aptamer sensor designed to enhance the sensitivity and specificity of LDL detection.
Methods
The study developed a fluorescent sensor using a copper-based metal-organic framework (Cu-MOF) and MoS₂ nanosheets. The sensor's performance was evaluated based on its ability to detect LDL in a range from 0.1 to 10,000 ng/mL. Specific parameters such as accuracy, precision, and reproducibility were assessed using human serum samples.
Results
The sensor achieved an ultra-low detection limit of 0.0789 ng/mL for LDL. It demonstrated a broad linear range of 0.1-10,000 ng/mL and showed relative errors between 0.09% and 3.00% in human serum samples. The relative standard deviations ranged from 1.24% to 3.74%, indicating good reproducibility.
Interpretation
The findings suggest that this sensor could significantly improve LDL monitoring compared to existing methods. While the statistical significance of the results is clear, the clinical relevance remains to be fully established, particularly regarding how these detection limits translate into meaningful patient outcomes. Potential confounds include the lack of long-term follow-up and validation in diverse populations.
Key findings
- broad linear range (0.1-10,000 ng/mL of LDL concentration)
- ultra-low detection limit (0.0789 ng/mL)
- relative errors of 0.09% ∼ 3.00%
- relative standard deviations of 1.24% ∼ 3.74%
Limitations
- Not reported in abstract.