An injectable hydroxyapatite microsphere filler loaded with GHK-Cu tripeptide for anti-Inflammatory and antioxidant.
The GHK-Cu@CMHA gel shows promise for reducing inflammation and enhancing antioxidant activity in soft tissue fillers, but further research is needed to confirm its clinical relevance.
Where it sits
this study against the rest of the ghk-cu corpusSummary and findings
This study evaluated an injectable filler combining hydroxyapatite microspheres (HAPs) and GHK-Cu for anti-inflammatory and antioxidant effects. The filler was tested in an LPS-induced inflammation model. Not reported in abstract.
Abstract
With the wide application of soft tissue fillers, implant material-induced inflammatory reactions have become a key factor affecting the therapeutic efficacy. This study developed an injectable filler with enhanced anti-inflammatory and antioxidant effects by adsorbing glycyl-L-histidyl-L-lysine copper complex (GHK-Cu) onto hydroxyapatite microspheres (HAPs), marking the first combination of HAPs and GHK-Cu to address inflammation caused by soft tissue fillers. GHK-Cu was successfully loaded onto HAPs by electrostatic adsorption. HAPs were then mixed with carboxymethyl cellulose (CMC), glycerol (GLY), and water to form GHK-Cu@CMHA gel. The study focus on the effective combination of HAPs as a carrier for sustained GHK-Cu delivery and the anti-inflammatory properties of GHK-Cu. GHK-Cu@CMHA exhibits sustained release properties for 7 days, which ensures prolonged therapeutic effects, minimizes peptide waste and reduces injection frequency, with good flowability and injectability. In the model of LPS-induced inflammation model in vivo and in vitro, GHK-Cu@CMHA gel reduced levels of inflammatory factors and Reactive oxygen species (ROS) levels decreased, while superoxide dismutase (SOD) activity was enhanced. In this process, H&E staining and Masson staining revealed significant collagen deposition. These findings further confirm that GHK-Cu@CMHA is a novel injectable soft tissue filler with good anti-inflammatory and antioxidant properties, which holds well potential for inflammation inhibition.
Background
The paper addresses the potential benefits of GHK-Cu, a tripeptide known for its biological activities, when delivered via an injectable hydroxyapatite microsphere filler. Previous studies have suggested that GHK-Cu may have anti-inflammatory and antioxidant effects, but the specific mechanisms and efficacy in a filler form remain unclear. This study aims to fill that gap by exploring the properties of this novel formulation.
Methods
Not reported in abstract.
Results
Not reported in abstract.
Interpretation
Not reported in abstract.
Key findings
- Not reported in abstract.
Limitations
- Not reported in abstract.