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Study 26 of 36HCG (Human Chorionic Gonadotropin) literaturebiorxiv-preprint · Observational2026

Peak LH/FSH ratio is associated with hCG response in prepubertal boys with micropenis

The peak LH/FSH ratio may help predict hCG response in prepubertal boys with micropenis, but its low specificity limits clinical utility.

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Where it sits

this study against the rest of the hcg (human chorionic gonadotropin) corpus
8
Preclinical
25
Observational · this one
0
Open-label
1
Randomised
2
Reviews

Summary and findings

The study investigated factors influencing hCG response in 92 prepubertal boys with micropenis, focusing on the peak LH/FSH ratio. A lower peak LH/FSH ratio was associated with a low hCG response. Multivariate logistic regression identified peak LH/FSH as an independent predictor of hCG response.

How much of this paper we could read: full text read (0.80). We had a clear abstract, so the summary below closely tracks the paper. What this means →
Peak LH/FSH independently predicted low hCG response (OR = 0.059, 95% CI: 0.003–0.579, P = 0.028).n=922026

Abstract

The authors’ words, as biorxiv-preprint supplied them

<title>Abstract</title> <p>Background Human chorionic gonadotropin (hCG) serves not only as a diagnostic stimulation test for evaluating testicular function but also as a key therapeutic modality for hypogonadotropic hypogonadism (HH). However, there are significant individual differences in hCG response among pediatric patients, and currently, there is a lack of effective clinical indicators to predict hCG response. The purpose of this study is to explore factors impacting hCG response in children with micropenis who are prepubertal. Methods This study enrolled prepubertal boys with micropenis and divided them into a low-response group and a normal-response group to compare clinical data between the two groups. Utilized multivariate logistic regression, correlation analysis, and receiver operating characteristic (ROC) curves to identify predictors of hCG response. Results A total of 92 prepubertal boys with micropenis were enrolled, including 20 with low hCG response and 72 with normal response. In comparison to the normal - response group,the low-response group demonstrated lower levels of peak luteinizing hormone ( LH ), a decreased ratio of peak LH to peak follicle - stimulating hormone ( FSH ) levels ( peak LH/FSH ), as well as reduced increases in testosterone ( ΔTT ), penile length ( ΔPL ), and relative change in penile length ( ΔPL/PL ) (P < 0.05). Multivariate logistic regression analysis indicated that peak LH/FSH (OR = 0.059, 95% CI: 0.003–0.579, P = 0.028) independently predicted a low hCG response. The peak LH/FSH was correlated positively with TT, ΔTT, ΔPL, and ΔPL/PL after hCG stimulation (P < 0.05). ROC curve analysis revealed that the area under the curve (AUC) for predicting low hCG response based on peak LH/FSH levels was 0.724 (95% CI: 0.595–0.854, P = 0.002), corresponding to an optimal cutoff value of 0.562, a sensitivity of 95.0%, and a specificity of 43.1%. Conclusion The peak LH/FSH ratio may serve as a potential predictive indicator of low hCG response in prepubertal children with micropenis. A lower peak LH/FSH ratio aids in identifying patients with micropenis who exhibit a low hCG response and guides subsequent treatment strategies.</p>

Background

Human chorionic gonadotropin (hCG) is used both diagnostically and therapeutically in hypogonadotropic hypogonadism, yet variability in patient response complicates treatment. This study addresses the need for predictive indicators of hCG response in prepubertal boys with micropenis, a condition often linked to hypogonadism. Understanding these predictors could enhance treatment personalization and outcomes.

Methods

The study included 92 prepubertal boys with micropenis, divided into low-response and normal-response groups based on hCG response. Multivariate logistic regression, correlation analysis, and ROC curves were employed to identify predictors of hCG response. The primary outcome was the peak LH/FSH ratio's ability to predict hCG response.

Results

The study found that the peak LH/FSH ratio was significantly lower in the low-response group (P < 0.05). Multivariate logistic regression showed that peak LH/FSH was an independent predictor of low hCG response (OR = 0.059, 95% CI: 0.003–0.579, P = 0.028). ROC analysis indicated an AUC of 0.724 for predicting low hCG response, with a sensitivity of 95.0% and specificity of 43.1% at a cutoff of 0.562.

Interpretation

The findings suggest that the peak LH/FSH ratio could serve as a predictive marker for hCG response in this population, though the low specificity limits its clinical application. Compared to prior literature, this study provides a quantifiable metric for predicting response, yet the clinical significance remains moderate due to the specificity issue. The study's focus on a narrow patient group and use of surrogate endpoints are notable limitations.

Key findings

  • 92 prepubertal boys with micropenis were enrolled.
  • 20 boys had a low hCG response, while 72 had a normal response.
  • Peak LH/FSH ratio was lower in the low-response group (P < 0.05).
  • Peak LH/FSH independently predicted low hCG response (OR = 0.059, 95% CI: 0.003–0.579, P = 0.028).
  • AUC for predicting low hCG response was 0.724 (95% CI: 0.595–0.854, P = 0.002).
  • Optimal cutoff for peak LH/FSH was 0.562 with sensitivity 95.0% and specificity 43.1%.

Limitations

  • Specific to prepubertal boys with micropenis.
  • Low specificity of peak LH/FSH ratio.
  • Single-site study.
  • Surrogate endpoints used.
  • Potential confounding variables not fully addressed.

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