Effect of Ebselen on Glucose and Redox Homoeostases in Diabetic Rats Exposed to Fenton Reagent and Crude Oil
Ebselen showed potential in reversing hyperglycemia and oxidative stress in diabetic rats exposed to pollutants, but further research is needed to confirm these effects in humans.
Where it sits
this study against the rest of the glutathione (gsh) corpusSummary and findings
The study measured the effects of Ebselen on glucose and redox homeostasis in diabetic rats exposed to Fenton reagent and crude oil. Fifty-six adult male Wistar rats were used, with diabetes induced via STZ (40 mg/kg) and treated with 10 mg/kg Ebselen for 30 days. Results indicated significant weight loss and hyperglycemia in diabetic rats, which were ameliorated by Ebselen treatment.
Abstract
<title>Abstract</title> <p>Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia resulting from impaired insulin secretion or insulin resistance. Its progression is strongly associated with oxidative stress–mediated cellular dysfunction. Emerging pollutants of environmental concern (POECs) may exacerbate redox imbalance in diabetic conditions through oxidative damage, organ dysfunction, and metabolic disturbances among others. Ebselen, a synthetic organoselenium compound with potent antioxidant, anti-inflammatory, and glutathione peroxidase (GPx)-mimetic activities. This study investigated the impact of some POECs, exemplified by Fenton reagent (FR) and Bonny light (BL) crude oil on the modulatory efficacy of Ebselen on blood sugar and redox status of diabetic rats. Fifty-six adult male Wistar rats (100–150 g) were assigned into eight groups (n = 7). Diabetes was induced via intraperitoneal injection of STZ (40 mg/kg) and rats were treated as followed: Group 1 (Normal rats); 2 (Diabetic rats); 3 (Normal rats + FR and BL crude oil mixture); 4 (Diabetic rats + FR and BL crude oil mixture); 5 (Normal rats + 10mg/kg ebselen); 6 (Diabetic rats + 10mg/kg ebselen); 7 (Normal rats + FR and BL crude oil mixture + 10mg/kg ebselen); 8 (Diabetic rats + FR and BL crude oil mixture + 10mg/kg ebselen) for 30 days. Rats were euthanised, and tissue samples were collected for biochemical assays. Results revealed that diabetic rats exhibited significant (p < 0.05) weight loss, hyperglycemia, elevated oxidative stress (increased TBARS, reduced DPPH scavenging activity and FRAP), reduced antioxidants (GPx, SOD, CAT, and GSH levels). Exposure to FR and BL crude oil further exacerbated these pathological changes. However, Ebselen administration significantly (p < 0.05) ameliorated hyperglycemia, restored body weight and elevated antioxidant status in the pollutant-exposed diabetic rats. Findings herein suggest that Ebselen may have potential therapeutic effect in pollutant-exacerbated diabetic conditions and could be considered as part of combinatorial therapy for diabetes upon further investigation.</p>