Cationic exchange SPE combined with triple quadrupole UHPLC-MS/MS for detection of GHRHs in urine samples.
The study presents a validated method for detecting GHRHs in urine with a limit of detection of 0.2 ng/mL, which may be useful for anti-doping screening.
Where it sits
this study against the rest of the cjc-1295 corpusSummary and findings
This study developed and validated a method for detecting growth hormone-releasing hormones (GHRHs) in urine samples, including CJC-1295. The method achieved a limit of detection (LOD) of 0.2 ng/mL and a limit of quantification (LOQ) of 0.6 ng/mL. The findings suggest that the method is suitable for routine screening in anti-doping laboratories.
Abstract
The use of growth hormone-releasing hormones (GHRHs) is prohibited in sports according to the regulations of the World Anti-Doping Agency (WADA). Considering the complexity of urine samples and the low concentrations at which these analytes should be detected, analyzing GHRHs is a challenging task. In most of the studies, GHRHs are analyzed using UHPLC-HRMS with an orbitrap. The present developed and validated method for some GHRHs (tesamorelin, CJC-1295, sermorelin (GRF 1-29), sermorelin (3-29)-NH<sub>2</sub>, somatorelin) is based on the triple quadrupole UHPLC/MS-MS method with solid phase extraction (SPE) with weak cation exchange and is able to detect concentrations as low as 0.2 ng/mL (LOD), a limit of quantification (LOQ) at 0.6 ng/mL, and linearity across the range of 0.1 ng/mL to 1.2 ng/mL. The present method developed by our doping control laboratory was validated according to WADA technical documents for selectivity, limit of detection (LOD), carryover, reliability of detection, stability and recovery. The results show that the method has adequate recoveries and sensitivity, hence, it can be employed for routine screening in anti-doping laboratories.
Background
The paper addresses the detection of growth hormone-releasing hormones (GHRHs) in urine, which is significant for understanding their role in various physiological processes. Prior methods for detecting GHRHs may have limitations in sensitivity and specificity. This study aims to improve detection methods, which could enhance research and clinical assessments involving GHRHs.
Methods
Not reported in abstract.
Results
Not reported in abstract.
Interpretation
Not reported in abstract.
Key findings
- Not reported in abstract.
Limitations
- Not reported in abstract.