Ultrasound-activated trilayer piezoelectric bio-adhesive for rapid repair and antibacterial treatment of gastrointestinal perforation.
This study presents a novel piezoelectric bio-adhesive that achieved full-thickness healing in rat models of gastrointestinal perforation within 15 days.
Where it sits
this study against the rest of the mazdutide (ibi362) corpusSummary and findings
This study investigates a trilayer piezoelectric bio-adhesive for gastrointestinal perforation repair in rat models. The bio-adhesive demonstrated full-thickness healing within 15 days and reduced bacterial burden in a cecal ligation and puncture model. No therapeutic claims are made.
Abstract
Gastrointestinal perforation (GIP) represents a critical abdominal emergency, the management of which hinges on prompt closure of the perforation and effective infection control. Currently, the application of biomaterials in the repair of acute GIP primarily focuses on rapid sealing and tissue repair, while still exhibiting certain limitations in antibacterial efficacy. To address this limitation, we developed a trilayer piezoelectric bio-adhesive consisting of an antibacterial piezoelectric layer, a conductive adhesive layer, and a a tissue-regenerative layer. The polyhydroxybutyrate (PHB) piezoelectric membrane loaded with silver-doped graphene oxide (Ag@GO) nanosheets, as the outermost layer, could generate the piezoelectric signals under ultrasound treatment, which were transmitted to the surrounding wound area through a tissue-adhesive poly(3-amino-4-methoxybenzoic acid)-gelatin (PAMB-G) hydrogel. Simultaneously, the innermost layer, prepared using a decellularized extracellular matrix (dECM) scaffold, provides a favorable microenvironment for tissue repair and vascular regeneration. <i>In vitro</i> experiments demonstrated that piezoelectric stimulation has dual functionality, facilitating tissue repair and exerting antibacterial effects, mainly because piezoelectric effects mediate Piezo1-dependent calcium influx, which promotes fibroblast activity and macrophage immunomodulation. <i>In vivo</i> experimental results demonstrated that under ultrasound (US) activation, this designed trilayer bio-adhesive achieved full-thickness healing within 15 days in rat models of gastric and small intestinal perforation. Furthermore, the bio-adhesive reduced the intraperitoneal bacterial burden in a cecal ligation and puncture (CLP) model and maintained stable leakage prevention and favorable tissue-healing capability in a pancreatic fistula model. This study presents an ultrasound-responsive trilayer piezoelectric biomaterial with rapid sealing, synergistic antibacterial activity, and tissue repair-promoting capabilities, offering a novel strategy and potential approach for treating gastrointestinal perforations.
Background
Gastrointestinal perforation (GIP) is a critical emergency requiring rapid intervention for closure and infection control. Existing biomaterials for GIP repair face challenges in antibacterial efficacy. This study introduces a novel trilayer piezoelectric bio-adhesive designed to enhance tissue repair and provide antibacterial effects, addressing limitations of current materials.
Methods
The study utilized rat models to evaluate the efficacy of a trilayer piezoelectric bio-adhesive. The bio-adhesive consists of an antibacterial piezoelectric layer, a conductive adhesive layer, and a tissue-regenerative layer. The primary outcome measure was the rate of full-thickness healing, assessed over a period of 15 days.
Results
Full-thickness healing was achieved within 15 days in the rat models of gastric and small intestinal perforation. The bio-adhesive also reduced bacterial burden in a cecal ligation and puncture model, although specific numeric data for bacterial reduction was not reported in abstract.
Interpretation
The findings suggest that the trilayer bio-adhesive may offer a promising approach for GIP repair, with potential benefits in antibacterial activity and tissue regeneration. However, the effect size and clinical significance in human applications remain uncertain due to the study's reliance on animal models and the absence of long-term follow-up data.
Key findings
- Full-thickness healing achieved within 15 days in rat models of gastric and small intestinal perforation.
- Reduced intraperitoneal bacterial burden in a cecal ligation and puncture model.
- Maintained stable leakage prevention and favorable tissue-healing capability in a pancreatic fistula model.
Limitations
- Based on rat models, which may not fully translate to humans.
- Lack of long-term follow-up data.
- Not reported in abstract.