Electroporation Delivery of Cas9 sgRNA Ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes.
Electroporation can achieve a 26% knock-in efficiency for gene editing in sheep IVF zygotes, highlighting the importance of optimizing delivery parameters.
Where it sits
this study against the rest of the ovagen (edl) corpusSummary and findings
This study investigated the use of electroporation for delivering CRISPR/Cas9 components to sheep IVF zygotes, focusing on the ACTG1 and MSTN genes. The research reported a knock-in efficiency of 26% for the MSTN gene. The study aimed to optimize electroporation parameters for improved gene editing outcomes.
Abstract
The utilization of electroporation for delivering CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development of genome-edited animals. In this study, our research focused on targeting the <i>ACTG1</i> and <i>MSTN</i> genes in sheep, revealing a threshold phenomenon in electroporation with a voltage tolerance in sheep in vitro fertilization (IVF) zygotes. Various poring voltages near 40 V and pulse durations were examined for electroporating sheep zygotes. The study concluded that stronger electric fields required shorter pulse durations to achieve the optimal conditions for high gene mutation rates and reasonable blastocyst development. This investigation also assessed the quality of Cas9/sgRNA ribonucleoprotein complexes (Cas9 RNPs) and their influence on genome editing efficiency in sheep early embryos. It was highlighted that pre-complexation of Cas9 proteins with single-guide RNA (sgRNA) before electroporation was essential for achieving a high mutation rate. The use of suitable electroporation parameters for sheep IVF zygotes led to significantly high mutation rates and heterozygote ratios. By delivering Cas9 RNPs and single-stranded oligodeoxynucleotides (ssODNs) to zygotes through electroporation, targeting the <i>MSTN</i> (Myostatin) gene, a knock-in efficiency of 26% was achieved. The successful generation of <i>MSTN</i>-modified lambs was demonstrated by delivering Cas9 RNPs into IVF zygotes via electroporation.